Construct: shRNA TRCN0000415705

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001170.1-498s21c1
DNA Barcode:
GGAGGAAGTTTCCGGTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AQP7 (364)
Gene Description:
aquaporin 7
Transcript:
RefSeq NM_001170.1 (NON-CURRENT)
Match location:
Position 498 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415705)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAGGAAGTTTCCGGTCTATG
Hairpin Sequence:
5'-CCGG-GGAGGAAGTTTCCGGTCTATG-CTCGAG-CATAGACCGGAAACTTCCTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAGGAAGTTTCCGGTCTATGCTCGAGCATAGACCGGAAACTTCCTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAGGAAGTTTCCGGTCTATGCTCGAGCATAGACCGGAAACTTCCTCC-3'

Other clones with same target sequence:

(none)