Construct: shRNA TRCN0000415725

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145249.2-357s21c1
DNA Barcode:
GGCTTCACCTCAGTAGGAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IFI27L1 (122509)
Gene Description:
interferon alpha inducible protein 27 like 1
Transcript:
RefSeq NM_145249.2 (NON-CURRENT)
Match location:
Position 357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415725)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTTCACCTCAGTAGGAATC
Hairpin Sequence:
5'-CCGG-GGCTTCACCTCAGTAGGAATC-CTCGAG-GATTCCTACTGAGGTGAAGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTTCACCTCAGTAGGAATCCTCGAGGATTCCTACTGAGGTGAAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTTCACCTCAGTAGGAATCCTCGAGGATTCCTACTGAGGTGAAGCC-3'

Other clones with same target sequence:

(none)