Construct: shRNA TRCN0000415794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023729.3-628s21c1
DNA Barcode:
GCTTGAACTGGGAGCTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asz1 (74068)
Gene Description:
ankyrin repeat, SAM and basic leucine zipper domain containing 1
Transcript:
RefSeq NM_023729.3 (CURRENT)
Match location:
Position 628 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGAACTGGGAGCTAATAA
Hairpin Sequence:
5'-CCGG-GCTTGAACTGGGAGCTAATAA-CTCGAG-TTATTAGCTCCCAGTTCAAGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGAACTGGGAGCTAATAACTCGAGTTATTAGCTCCCAGTTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGAACTGGGAGCTAATAACTCGAGTTATTAGCTCCCAGTTCAAGC-3'

Other clones with same target sequence:

(none)