Construct: shRNA TRCN0000415846

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152525.5-1930s21c1
DNA Barcode:
GTTCGCTCATTTGTGATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C2CD6 (151254)
Gene Description:
C2 calcium dependent domain containing 6
Transcript:
RefSeq NM_152525.5 (NON-CURRENT)
Match location:
Position 1930 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415846)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCGCTCATTTGTGATATTA
Hairpin Sequence:
5'-CCGG-GTTCGCTCATTTGTGATATTA-CTCGAG-TAATATCACAAATGAGCGAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCGCTCATTTGTGATATTACTCGAGTAATATCACAAATGAGCGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCGCTCATTTGTGATATTACTCGAGTAATATCACAAATGAGCGAAC-3'

Other clones with same target sequence:

(none)