Construct: shRNA TRCN0000415862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020384.3-1338s21c1
DNA Barcode:
AGGACTCAGAGGATCCCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLDN2 (9075)
Gene Description:
claudin 2
Transcript:
RefSeq NM_020384.3 (NON-CURRENT)
Match location:
Position 1338 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGACTCAGAGGATCCCTTTG
Hairpin Sequence:
5'-CCGG-AGGACTCAGAGGATCCCTTTG-CTCGAG-CAAAGGGATCCTCTGAGTCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGACTCAGAGGATCCCTTTGCTCGAGCAAAGGGATCCTCTGAGTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGACTCAGAGGATCCCTTTGCTCGAGCAAAGGGATCCTCTGAGTCCT-3'

Other clones with same target sequence:

(none)