Construct: shRNA TRCN0000415961

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182524.2-2158s21c1
DNA Barcode:
AGAATATTGCTCCCATATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF595 (152687)
Gene Description:
zinc finger protein 595
Transcript:
RefSeq NM_182524.2 (NON-CURRENT)
Match location:
Position 2158 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415961)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATATTGCTCCCATATAAA
Hairpin Sequence:
5'-CCGG-AGAATATTGCTCCCATATAAA-CTCGAG-TTTATATGGGAGCAATATTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATATTGCTCCCATATAAACTCGAGTTTATATGGGAGCAATATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATATTGCTCCCATATAAACTCGAGTTTATATGGGAGCAATATTCT-3'

Other clones with same target sequence:

(none)