Construct: shRNA TRCN0000415992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005329.2-1136s21c1
DNA Barcode:
TTGGCTACCGAACTAAGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HAS3 (3038)
Gene Description:
hyaluronan synthase 3
Transcript:
RefSeq NM_005329.2 (CURRENT)
Match location:
Position 1136 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000415992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGCTACCGAACTAAGTATA
Hairpin Sequence:
5'-CCGG-TTGGCTACCGAACTAAGTATA-CTCGAG-TATACTTAGTTCGGTAGCCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGCTACCGAACTAAGTATACTCGAGTATACTTAGTTCGGTAGCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGCTACCGAACTAAGTATACTCGAGTATACTTAGTTCGGTAGCCAA-3'

Other clones with same target sequence:

(none)