Construct: shRNA TRCN0000416025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172880.2-1237s21c1
DNA Barcode:
TGAATGTGGCCAACCCAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmprss11e (243084)
Gene Description:
transmembrane protease, serine 11e
Transcript:
RefSeq NM_172880.2 (CURRENT)
Match location:
Position 1237 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAATGTGGCCAACCCAATAA
Hairpin Sequence:
5'-CCCG-TGAATGTGGCCAACCCAATAA-CTCGAG-TTATTGGGTTGGCCACATTCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAATGTGGCCAACCCAATAACTCGAGTTATTGGGTTGGCCACATTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAATGTGGCCAACCCAATAACTCGAGTTATTGGGTTGGCCACATTCA-3'

Other clones with same target sequence:

(none)