Construct: shRNA TRCN0000416054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146041.2-270s21c1
DNA Barcode:
AGAATCCTCAGGCTCATATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gmds (218138)
Gene Description:
GDP-mannose 4, 6-dehydratase
Transcript:
RefSeq NM_146041.2 (CURRENT)
Match location:
Position 270 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATCCTCAGGCTCATATTG
Hairpin Sequence:
5'-CCGG-AGAATCCTCAGGCTCATATTG-CTCGAG-CAATATGAGCCTGAGGATTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATCCTCAGGCTCATATTGCTCGAGCAATATGAGCCTGAGGATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATCCTCAGGCTCATATTGCTCGAGCAATATGAGCCTGAGGATTCT-3'

Other clones with same target sequence:

(none)