Construct: shRNA TRCN0000416114

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011144.6-2138s21c1
DNA Barcode:
GCACTCTACGTTGCGTTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppara (19013)
Gene Description:
peroxisome proliferator activated receptor alpha
Transcript:
RefSeq NM_011144.6 (CURRENT)
Match location:
Position 2138 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416114)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTCTACGTTGCGTTTATA
Hairpin Sequence:
5'-CCGG-GCACTCTACGTTGCGTTTATA-CTCGAG-TATAAACGCAACGTAGAGTGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTCTACGTTGCGTTTATACTCGAGTATAAACGCAACGTAGAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTCTACGTTGCGTTTATACTCGAGTATAAACGCAACGTAGAGTGC-3'

Other clones with same target sequence:

(none)