Construct: shRNA TRCN0000416163

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025189.2-619s21c1
DNA Barcode:
CAACTACCCAGAGTGAAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF430 (80264)
Gene Description:
zinc finger protein 430
Transcript:
RefSeq NM_025189.2 (NON-CURRENT)
Match location:
Position 619 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416163)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTACCCAGAGTGAAATAT
Hairpin Sequence:
5'-CCGG-CAACTACCCAGAGTGAAATAT-CTCGAG-ATATTTCACTCTGGGTAGTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTACCCAGAGTGAAATATCTCGAGATATTTCACTCTGGGTAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTACCCAGAGTGAAATATCTCGAGATATTTCACTCTGGGTAGTTG-3'

Other clones with same target sequence:

(none)