Construct: shRNA TRCN0000416189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006506.2-646s21c1
DNA Barcode:
ACAAGCAATCCGCAGTTTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASA2 (5922)
Gene Description:
RAS p21 protein activator 2
Transcript:
RefSeq NM_006506.2 (NON-CURRENT)
Match location:
Position 646 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAGCAATCCGCAGTTTAAT
Hairpin Sequence:
5'-CCGG-ACAAGCAATCCGCAGTTTAAT-CTCGAG-ATTAAACTGCGGATTGCTTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAGCAATCCGCAGTTTAATCTCGAGATTAAACTGCGGATTGCTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAGCAATCCGCAGTTTAATCTCGAGATTAAACTGCGGATTGCTTGT-3'

Other clones with same target sequence:

(none)