Construct: shRNA TRCN0000416201

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004001.3-956s21c1
DNA Barcode:
CACAATCACCTATTCACTTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FCGR2B (2213)
Gene Description:
Fc fragment of IgG receptor IIb
Transcript:
RefSeq NM_004001.3 (NON-CURRENT)
Match location:
Position 956 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416201)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAATCACCTATTCACTTCT
Hairpin Sequence:
5'-CCGG-CACAATCACCTATTCACTTCT-CTCGAG-AGAAGTGAATAGGTGATTGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAATCACCTATTCACTTCTCTCGAGAGAAGTGAATAGGTGATTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAATCACCTATTCACTTCTCTCGAGAGAAGTGAATAGGTGATTGTG-3'

Other clones with same target sequence:

(none)