Construct: shRNA TRCN0000416210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183027.2-361s21c1
DNA Barcode:
CTAGAGATTGTTCATCGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ap1s3 (252903)
Gene Description:
adaptor-related protein complex AP-1, sigma 3
Transcript:
RefSeq NM_183027.2 (CURRENT)
Match location:
Position 361 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGAGATTGTTCATCGTTAT
Hairpin Sequence:
5'-CCGG-CTAGAGATTGTTCATCGTTAT-CTCGAG-ATAACGATGAACAATCTCTAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGAGATTGTTCATCGTTATCTCGAGATAACGATGAACAATCTCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGAGATTGTTCATCGTTATCTCGAGATAACGATGAACAATCTCTAG-3'

Other clones with same target sequence:

(none)