Construct: shRNA TRCN0000416218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031938.4-359s21c1
DNA Barcode:
ATGGCTCTCTACTTCGAATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCO2 (83875)
Gene Description:
beta-carotene oxygenase 2
Transcript:
RefSeq NM_031938.4 (NON-CURRENT)
Match location:
Position 359 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGCTCTCTACTTCGAATTG
Hairpin Sequence:
5'-CCGG-ATGGCTCTCTACTTCGAATTG-CTCGAG-CAATTCGAAGTAGAGAGCCAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGCTCTCTACTTCGAATTGCTCGAGCAATTCGAAGTAGAGAGCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGCTCTCTACTTCGAATTGCTCGAGCAATTCGAAGTAGAGAGCCAT-3'

Other clones with same target sequence:

(none)