Construct: shRNA TRCN0000416235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025256.5-2931s21c1
DNA Barcode:
AGATTGAGCCTCCGCTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EHMT2 (10919)
Gene Description:
euchromatic histone lysine methyltransferase 2
Transcript:
RefSeq NM_025256.5 (NON-CURRENT)
Match location:
Position 2931 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATTGAGCCTCCGCTGATTT
Hairpin Sequence:
5'-CCGG-AGATTGAGCCTCCGCTGATTT-CTCGAG-AAATCAGCGGAGGCTCAATCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATTGAGCCTCCGCTGATTTCTCGAGAAATCAGCGGAGGCTCAATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATTGAGCCTCCGCTGATTTCTCGAGAAATCAGCGGAGGCTCAATCT-3'

Other clones with same target sequence:

(none)