Construct: shRNA TRCN0000416245

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024753.3-2896s21c1
DNA Barcode:
GTTGGAACTGGCACGATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTC21B (79809)
Gene Description:
tetratricopeptide repeat domain 21B
Transcript:
RefSeq NM_024753.3 (NON-CURRENT)
Match location:
Position 2896 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416245)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGGAACTGGCACGATTATA
Hairpin Sequence:
5'-CCGG-GTTGGAACTGGCACGATTATA-CTCGAG-TATAATCGTGCCAGTTCCAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGGAACTGGCACGATTATACTCGAGTATAATCGTGCCAGTTCCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGGAACTGGCACGATTATACTCGAGTATAATCGTGCCAGTTCCAAC-3'

Other clones with same target sequence:

(none)