Construct: shRNA TRCN0000416327

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006449.3-1630s21c1
DNA Barcode:
CTCATCAAGGGAAAGACTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDC42EP3 (10602)
Gene Description:
CDC42 effector protein 3
Transcript:
RefSeq NM_006449.3 (NON-CURRENT)
Match location:
Position 1630 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416327)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATCAAGGGAAAGACTAAG
Hairpin Sequence:
5'-CCGG-CTCATCAAGGGAAAGACTAAG-CTCGAG-CTTAGTCTTTCCCTTGATGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATCAAGGGAAAGACTAAGCTCGAGCTTAGTCTTTCCCTTGATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATCAAGGGAAAGACTAAGCTCGAGCTTAGTCTTTCCCTTGATGAG-3'

Other clones with same target sequence:

(none)