Construct: shRNA TRCN0000416379

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025883.3-1202s21c1
DNA Barcode:
CAACTGGATGAAGTCTTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zdhhc6 (66980)
Gene Description:
zinc finger, DHHC domain containing 6
Transcript:
RefSeq NM_025883.3 (CURRENT)
Match location:
Position 1202 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416379)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTGGATGAAGTCTTCATT
Hairpin Sequence:
5'-CCGG-CAACTGGATGAAGTCTTCATT-CTCGAG-AATGAAGACTTCATCCAGTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTGGATGAAGTCTTCATTCTCGAGAATGAAGACTTCATCCAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTGGATGAAGTCTTCATTCTCGAGAATGAAGACTTCATCCAGTTG-3'

Other clones with same target sequence:

(none)