Construct: shRNA TRCN0000416494

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080605.3-473s21c1
DNA Barcode:
CCTTCGAGTTCGTGCTCAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B3GALT6 (126792)
Gene Description:
beta-1,3-galactosyltransferase 6
Transcript:
RefSeq NM_080605.3 (NON-CURRENT)
Match location:
Position 473 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416494)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCGAGTTCGTGCTCAAGG
Hairpin Sequence:
5'-CCGG-CCTTCGAGTTCGTGCTCAAGG-CTCGAG-CCTTGAGCACGAACTCGAAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCGAGTTCGTGCTCAAGGCTCGAGCCTTGAGCACGAACTCGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCGAGTTCGTGCTCAAGGCTCGAGCCTTGAGCACGAACTCGAAGG-3'

Other clones with same target sequence:

(none)