Construct: shRNA TRCN0000416506

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152663.3-1360s21c1
DNA Barcode:
GCCATAGTTTGGGTTATAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RALGPS2 (55103)
Gene Description:
Ral GEF with PH domain and SH3 binding motif 2
Transcript:
RefSeq NM_152663.3 (NON-CURRENT)
Match location:
Position 1360 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416506)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATAGTTTGGGTTATAATT
Hairpin Sequence:
5'-CCGG-GCCATAGTTTGGGTTATAATT-CTCGAG-AATTATAACCCAAACTATGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATAGTTTGGGTTATAATTCTCGAGAATTATAACCCAAACTATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATAGTTTGGGTTATAATTCTCGAGAATTATAACCCAAACTATGGC-3'

Other clones with same target sequence:

(none)