Construct: shRNA TRCN0000416512

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080387.4-1138s21c1
DNA Barcode:
TATGGAAGAATAGCGTGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLEC4D (338339)
Gene Description:
C-type lectin domain family 4 member D
Transcript:
RefSeq NM_080387.4 (NON-CURRENT)
Match location:
Position 1138 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416512)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGGAAGAATAGCGTGAATA
Hairpin Sequence:
5'-CCGG-TATGGAAGAATAGCGTGAATA-CTCGAG-TATTCACGCTATTCTTCCATA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGGAAGAATAGCGTGAATACTCGAGTATTCACGCTATTCTTCCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGGAAGAATAGCGTGAATACTCGAGTATTCACGCTATTCTTCCATA-3'

Other clones with same target sequence:

(none)