Construct: shRNA TRCN0000416542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020748.2-3945s21c1
DNA Barcode:
ACACATGTAAGAAACTCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INTS2 (57508)
Gene Description:
integrator complex subunit 2
Transcript:
RefSeq NM_020748.2 (NON-CURRENT)
Match location:
Position 3945 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACATGTAAGAAACTCATTA
Hairpin Sequence:
5'-CCGG-ACACATGTAAGAAACTCATTA-CTCGAG-TAATGAGTTTCTTACATGTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACATGTAAGAAACTCATTACTCGAGTAATGAGTTTCTTACATGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACATGTAAGAAACTCATTACTCGAGTAATGAGTTTCTTACATGTGT-3'

Other clones with same target sequence:

(none)