Construct: shRNA TRCN0000416579

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004051.1-1021s21c1
DNA Barcode:
GTAGGTGGCGCTCGTTCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GPRASP2 (114928)
Gene Description:
G protein-coupled receptor associated sorting protein 2
Transcript:
RefSeq NM_001004051.1 (NON-CURRENT)
Match location:
Position 1021 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416579)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGGTGGCGCTCGTTCTAAA
Hairpin Sequence:
5'-CCGG-GTAGGTGGCGCTCGTTCTAAA-CTCGAG-TTTAGAACGAGCGCCACCTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGGTGGCGCTCGTTCTAAACTCGAGTTTAGAACGAGCGCCACCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGGTGGCGCTCGTTCTAAACTCGAGTTTAGAACGAGCGCCACCTAC-3'

Other clones with same target sequence:

(none)