Construct: shRNA TRCN0000416640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000902.3-2563s21c1
DNA Barcode:
CTAAGGTCTATCAAGTCAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MME (4311)
Gene Description:
membrane metalloendopeptidase
Transcript:
RefSeq NM_000902.3 (NON-CURRENT)
Match location:
Position 2563 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAGGTCTATCAAGTCAATC
Hairpin Sequence:
5'-CCGG-CTAAGGTCTATCAAGTCAATC-CTCGAG-GATTGACTTGATAGACCTTAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAGGTCTATCAAGTCAATCCTCGAGGATTGACTTGATAGACCTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAGGTCTATCAAGTCAATCCTCGAGGATTGACTTGATAGACCTTAG-3'

Other clones with same target sequence:

(none)