Construct: shRNA TRCN0000416678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015600.3-1049s21c1
DNA Barcode:
CTATTACAAGTAGTGGAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABHD12 (26090)
Gene Description:
abhydrolase domain containing 12
Transcript:
RefSeq NM_015600.3 (NON-CURRENT)
Match location:
Position 1049 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATTACAAGTAGTGGAATTA
Hairpin Sequence:
5'-CCGG-CTATTACAAGTAGTGGAATTA-CTCGAG-TAATTCCACTACTTGTAATAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATTACAAGTAGTGGAATTACTCGAGTAATTCCACTACTTGTAATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATTACAAGTAGTGGAATTACTCGAGTAATTCCACTACTTGTAATAG-3'

Other clones with same target sequence:

(none)