Construct: shRNA TRCN0000416722

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014813.1-1243s21c1
DNA Barcode:
CATATTGCTGATGGTGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRIG2 (9860)
Gene Description:
leucine rich repeats and immunoglobulin like domains 2
Transcript:
RefSeq NM_014813.1 (NON-CURRENT)
Match location:
Position 1243 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416722)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATATTGCTGATGGTGTATTT
Hairpin Sequence:
5'-CCGG-CATATTGCTGATGGTGTATTT-CTCGAG-AAATACACCATCAGCAATATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATATTGCTGATGGTGTATTTCTCGAGAAATACACCATCAGCAATATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATATTGCTGATGGTGTATTTCTCGAGAAATACACCATCAGCAATATG-3'

Other clones with same target sequence:

(none)