Construct: shRNA TRCN0000416747

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173011.2-1230s21c1
DNA Barcode:
GATGGGAACCAGGACCTTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Idh2 (269951)
Gene Description:
isocitrate dehydrogenase 2 (NADP+), mitochondrial
Transcript:
RefSeq NM_173011.2 (CURRENT)
Match location:
Position 1230 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416747)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGGAACCAGGACCTTATC
Hairpin Sequence:
5'-CCGG-GATGGGAACCAGGACCTTATC-CTCGAG-GATAAGGTCCTGGTTCCCATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGGAACCAGGACCTTATCCTCGAGGATAAGGTCCTGGTTCCCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGGAACCAGGACCTTATCCTCGAGGATAAGGTCCTGGTTCCCATC-3'

Other clones with same target sequence:

(none)