Construct: shRNA TRCN0000416765

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000083.2-1090s21c1
DNA Barcode:
ACCAATTTCCGAATGGATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLCN1 (1180)
Gene Description:
chloride voltage-gated channel 1
Transcript:
RefSeq NM_000083.2 (NON-CURRENT)
Match location:
Position 1090 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416765)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAATTTCCGAATGGATTTC
Hairpin Sequence:
5'-CCGG-ACCAATTTCCGAATGGATTTC-CTCGAG-GAAATCCATTCGGAAATTGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAATTTCCGAATGGATTTCCTCGAGGAAATCCATTCGGAAATTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAATTTCCGAATGGATTTCCTCGAGGAAATCCATTCGGAAATTGGT-3'

Other clones with same target sequence:

(none)