Construct: shRNA TRCN0000416847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152453.2-586s21c1
DNA Barcode:
AGATCAAGCCCTCTACATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMCO5A (145942)
Gene Description:
transmembrane and coiled-coil domains 5A
Transcript:
RefSeq NM_152453.2 (NON-CURRENT)
Match location:
Position 586 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATCAAGCCCTCTACATAAA
Hairpin Sequence:
5'-CCGG-AGATCAAGCCCTCTACATAAA-CTCGAG-TTTATGTAGAGGGCTTGATCT-TTTTTTTGAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATCAAGCCCTCTACATAAACTCGAGTTTATGTAGAGGGCTTGATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATCAAGCCCTCTACATAAACTCGAGTTTATGTAGAGGGCTTGATCT-3'

Other clones with same target sequence:

(none)