Construct: shRNA TRCN0000416856

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005852.2-249s21c1
DNA Barcode:
GCCACAAGTAATAAACATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TXLNGY (246126)
Gene Description:
taxilin gamma pseudogene, Y-linked
Transcript:
RefSeq NM_001005852.2 (NON-CURRENT)
Match location:
Position 249 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416856)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACAAGTAATAAACATTTA
Hairpin Sequence:
5'-CCGG-GCCACAAGTAATAAACATTTA-CTCGAG-TAAATGTTTATTACTTGTGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACAAGTAATAAACATTTACTCGAGTAAATGTTTATTACTTGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACAAGTAATAAACATTTACTCGAGTAAATGTTTATTACTTGTGGC-3'

Other clones with same target sequence:

(none)