Construct: shRNA TRCN0000416866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_205548.1-345s21c1
DNA Barcode:
GAACACAGCCAGCCAATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM151B (167555)
Gene Description:
family with sequence similarity 151 member B
Transcript:
RefSeq NM_205548.1 (NON-CURRENT)
Match location:
Position 345 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACACAGCCAGCCAATTATG
Hairpin Sequence:
5'-CCGG-GAACACAGCCAGCCAATTATG-CTCGAG-CATAATTGGCTGGCTGTGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACACAGCCAGCCAATTATGCTCGAGCATAATTGGCTGGCTGTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACACAGCCAGCCAATTATGCTCGAGCATAATTGGCTGGCTGTGTTC-3'

Other clones with same target sequence:

(none)