Construct: shRNA TRCN0000416869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015157.2-3469s21c1
DNA Barcode:
AGAAGGGACAACAAGTGATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHLDB1 (23187)
Gene Description:
pleckstrin homology like domain family B member 1
Transcript:
RefSeq NM_015157.2 (NON-CURRENT)
Match location:
Position 3469 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGGGACAACAAGTGATTG
Hairpin Sequence:
5'-CCGG-AGAAGGGACAACAAGTGATTG-CTCGAG-CAATCACTTGTTGTCCCTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGGGACAACAAGTGATTGCTCGAGCAATCACTTGTTGTCCCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGGGACAACAAGTGATTGCTCGAGCAATCACTTGTTGTCCCTTCT-3'

Other clones with same target sequence:

(none)