Construct: shRNA TRCN0000416929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011995.4-12425s21c1
DNA Barcode:
GATTCGATGGAGGATCCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pclo (26875)
Gene Description:
piccolo (presynaptic cytomatrix protein)
Transcript:
RefSeq NM_011995.4 (CURRENT)
Match location:
Position 12425 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTCGATGGAGGATCCTTAT
Hairpin Sequence:
5'-CCGG-GATTCGATGGAGGATCCTTAT-CTCGAG-ATAAGGATCCTCCATCGAATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTCGATGGAGGATCCTTATCTCGAGATAAGGATCCTCCATCGAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTCGATGGAGGATCCTTATCTCGAGATAAGGATCCTCCATCGAATC-3'

Other clones with same target sequence:

(none)