Construct: shRNA TRCN0000416935
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_000306.2-805s21c1
- DNA Barcode:
- ATGCTCTGGAGAGACACTTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- POU1F1 (5449)
- Gene Description:
- POU class 1 homeobox 1
- Transcript:
- RefSeq NM_000306.2 (NON-CURRENT)
- Match location:
- Position 805 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ATGCTCTGGAGAGACACTTTG
- Hairpin Sequence:
-
5'-CCGG-ATGCTCTGGAGAGACACTTTG-CTCGAG-CAAAGTGTCTCTCCAGAGCAT-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGATGCTCTGGAGAGACACTTTGCTCGAGCAAAGTGTCTCTCCAGAGCATTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAATGCTCTGGAGAGACACTTTGCTCGAGCAAAGTGTCTCTCCAGAGCAT-3'
Other clones with same target sequence:
(none)
