Construct: shRNA TRCN0000416936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000531.5-674s21c1
DNA Barcode:
GAAGCATCCATCCCAATTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OTC (5009)
Gene Description:
ornithine carbamoyltransferase
Transcript:
RefSeq NM_000531.5 (NON-CURRENT)
Match location:
Position 674 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000416936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCATCCATCCCAATTATC
Hairpin Sequence:
5'-CCGG-GAAGCATCCATCCCAATTATC-CTCGAG-GATAATTGGGATGGATGCTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCATCCATCCCAATTATCCTCGAGGATAATTGGGATGGATGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCATCCATCCCAATTATCCTCGAGGATAATTGGGATGGATGCTTC-3'

Other clones with same target sequence:

(none)