Construct: shRNA TRCN0000417033

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138361.3-765s21c1
DNA Barcode:
TCCAGACTCTCAATGTTAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRSAM1 (90678)
Gene Description:
leucine rich repeat and sterile alpha motif containing 1
Transcript:
RefSeq NM_138361.3 (NON-CURRENT)
Match location:
Position 765 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417033)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAGACTCTCAATGTTAAAG
Hairpin Sequence:
5'-CCGG-TCCAGACTCTCAATGTTAAAG-CTCGAG-CTTTAACATTGAGAGTCTGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAGACTCTCAATGTTAAAGCTCGAGCTTTAACATTGAGAGTCTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAGACTCTCAATGTTAAAGCTCGAGCTTTAACATTGAGAGTCTGGA-3'

Other clones with same target sequence:

(none)