Construct: shRNA TRCN0000417096

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032169.4-756s21c1
DNA Barcode:
GGTCGAATCTTCCGTGATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACAD11 (84129)
Gene Description:
acyl-CoA dehydrogenase family member 11
Transcript:
RefSeq NM_032169.4 (NON-CURRENT)
Match location:
Position 756 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417096)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCGAATCTTCCGTGATTTA
Hairpin Sequence:
5'-CCGG-GGTCGAATCTTCCGTGATTTA-CTCGAG-TAAATCACGGAAGATTCGACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCGAATCTTCCGTGATTTACTCGAGTAAATCACGGAAGATTCGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCGAATCTTCCGTGATTTACTCGAGTAAATCACGGAAGATTCGACC-3'

Other clones with same target sequence:

(none)