Construct: shRNA TRCN0000417108

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010598.2-425s21c1
DNA Barcode:
TGGTATTAGGGAACATCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnab2 (16498)
Gene Description:
potassium voltage-gated channel, shaker-related subfamily, beta member 2
Transcript:
RefSeq NM_010598.2 (NON-CURRENT)
Match location:
Position 425 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417108)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTATTAGGGAACATCATTA
Hairpin Sequence:
5'-CCGG-TGGTATTAGGGAACATCATTA-CTCGAG-TAATGATGTTCCCTAATACCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTATTAGGGAACATCATTACTCGAGTAATGATGTTCCCTAATACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTATTAGGGAACATCATTACTCGAGTAATGATGTTCCCTAATACCA-3'

Other clones with same target sequence:

(none)