Construct: shRNA TRCN0000417113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172683.3-3480s21c1
DNA Barcode:
AGTTTGTGCAGCGGCAGATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pogz (229584)
Gene Description:
pogo transposable element with ZNF domain
Transcript:
RefSeq NM_172683.3 (CURRENT)
Match location:
Position 3480 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTTGTGCAGCGGCAGATTC
Hairpin Sequence:
5'-CCGG-AGTTTGTGCAGCGGCAGATTC-CTCGAG-GAATCTGCCGCTGCACAAACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTTGTGCAGCGGCAGATTCCTCGAGGAATCTGCCGCTGCACAAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTTGTGCAGCGGCAGATTCCTCGAGGAATCTGCCGCTGCACAAACT-3'

Other clones with same target sequence:

(none)