Construct: shRNA TRCN0000417139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005922.2-460s21c1
DNA Barcode:
GCCAGCCAGTCGGTCTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K4 (4216)
Gene Description:
mitogen-activated protein kinase kinase kinase 4
Transcript:
RefSeq NM_005922.2 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGCCAGTCGGTCTAATTT
Hairpin Sequence:
5'-CCGG-GCCAGCCAGTCGGTCTAATTT-CTCGAG-AAATTAGACCGACTGGCTGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGCCAGTCGGTCTAATTTCTCGAGAAATTAGACCGACTGGCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGCCAGTCGGTCTAATTTCTCGAGAAATTAGACCGACTGGCTGGC-3'

Other clones with same target sequence:

(none)