Construct: shRNA TRCN0000417311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182609.2-380s21c1
DNA Barcode:
TTATACCATCTGGTGATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF677 (342926)
Gene Description:
zinc finger protein 677
Transcript:
RefSeq NM_182609.2 (NON-CURRENT)
Match location:
Position 380 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTATACCATCTGGTGATATTA
Hairpin Sequence:
5'-CCGG-TTATACCATCTGGTGATATTA-CTCGAG-TAATATCACCAGATGGTATAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTATACCATCTGGTGATATTACTCGAGTAATATCACCAGATGGTATAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTATACCATCTGGTGATATTACTCGAGTAATATCACCAGATGGTATAA-3'

Other clones with same target sequence:

(none)