Construct: shRNA TRCN0000417372

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024563.3-639s21c1
DNA Barcode:
TCTACAGACCCTACTATAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C5orf23 (79614)
Gene Description:
chromosome 5 open reading frame 23
Transcript:
RefSeq NM_024563.3 (NON-CURRENT)
Match location:
Position 639 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417372)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTACAGACCCTACTATAATT
Hairpin Sequence:
5'-CCGG-TCTACAGACCCTACTATAATT-CTCGAG-AATTATAGTAGGGTCTGTAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTACAGACCCTACTATAATTCTCGAGAATTATAGTAGGGTCTGTAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTACAGACCCTACTATAATTCTCGAGAATTATAGTAGGGTCTGTAGA-3'

Other clones with same target sequence:

(none)