Construct: shRNA TRCN0000417440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017752.2-1689s21c1
DNA Barcode:
TACTAATCCTGACTATTATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D8B (54885)
Gene Description:
TBC1 domain family member 8B
Transcript:
RefSeq NM_017752.2 (NON-CURRENT)
Match location:
Position 1689 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTAATCCTGACTATTATAC
Hairpin Sequence:
5'-CCGG-TACTAATCCTGACTATTATAC-CTCGAG-GTATAATAGTCAGGATTAGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTAATCCTGACTATTATACCTCGAGGTATAATAGTCAGGATTAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTAATCCTGACTATTATACCTCGAGGTATAATAGTCAGGATTAGTA-3'

Other clones with same target sequence:

(none)