Construct: shRNA TRCN0000417482

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001979.4-1002s21c1
DNA Barcode:
ATATTGCATGGAAGTGTTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHX2 (2053)
Gene Description:
epoxide hydrolase 2
Transcript:
RefSeq NM_001979.4 (NON-CURRENT)
Match location:
Position 1002 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417482)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATTGCATGGAAGTGTTATG
Hairpin Sequence:
5'-CCGG-ATATTGCATGGAAGTGTTATG-CTCGAG-CATAACACTTCCATGCAATAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATTGCATGGAAGTGTTATGCTCGAGCATAACACTTCCATGCAATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATTGCATGGAAGTGTTATGCTCGAGCATAACACTTCCATGCAATAT-3'

Other clones with same target sequence:

(none)