Construct: shRNA TRCN0000417564

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014160.3-375s21c1
DNA Barcode:
TTGGTTCTTAGAGACCGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MKRN2 (23609)
Gene Description:
makorin ring finger protein 2
Transcript:
RefSeq NM_014160.3 (NON-CURRENT)
Match location:
Position 375 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417564)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGTTCTTAGAGACCGAAAT
Hairpin Sequence:
5'-CCGG-TTGGTTCTTAGAGACCGAAAT-CTCGAG-ATTTCGGTCTCTAAGAACCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGTTCTTAGAGACCGAAATCTCGAGATTTCGGTCTCTAAGAACCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGTTCTTAGAGACCGAAATCTCGAGATTTCGGTCTCTAAGAACCAA-3'

Other clones with same target sequence:

(none)