Construct: shRNA TRCN0000417578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016369.3-1195s21c1
DNA Barcode:
TAAACCCATTGATGATCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLDN18 (51208)
Gene Description:
claudin 18
Transcript:
RefSeq NM_016369.3 (NON-CURRENT)
Match location:
Position 1195 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAACCCATTGATGATCTATT
Hairpin Sequence:
5'-CCGG-TAAACCCATTGATGATCTATT-CTCGAG-AATAGATCATCAATGGGTTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAACCCATTGATGATCTATTCTCGAGAATAGATCATCAATGGGTTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAACCCATTGATGATCTATTCTCGAGAATAGATCATCAATGGGTTTA-3'

Other clones with same target sequence:

(none)