Construct: shRNA TRCN0000417697

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021026.2-1222s21c1
DNA Barcode:
AGGAACGCTCTACTCGGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RFPL1 (5988)
Gene Description:
ret finger protein like 1
Transcript:
RefSeq NM_021026.2 (CURRENT)
Match location:
Position 1222 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417697)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAACGCTCTACTCGGTAAA
Hairpin Sequence:
5'-CCGG-AGGAACGCTCTACTCGGTAAA-CTCGAG-TTTACCGAGTAGAGCGTTCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAACGCTCTACTCGGTAAACTCGAGTTTACCGAGTAGAGCGTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAACGCTCTACTCGGTAAACTCGAGTTTACCGAGTAGAGCGTTCCT-3'

Other clones with same target sequence:

(none)