Construct: shRNA TRCN0000417770

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008238.1-1099s21c1
DNA Barcode:
TCCGGAAGTTCCTCTCGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Foxn1 (15218)
Gene Description:
forkhead box N1
Transcript:
RefSeq NM_008238.1 (NON-CURRENT)
Match location:
Position 1099 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417770)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGGAAGTTCCTCTCGAAAG
Hairpin Sequence:
5'-CCGG-TCCGGAAGTTCCTCTCGAAAG-CTCGAG-CTTTCGAGAGGAACTTCCGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGGAAGTTCCTCTCGAAAGCTCGAGCTTTCGAGAGGAACTTCCGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGGAAGTTCCTCTCGAAAGCTCGAGCTTTCGAGAGGAACTTCCGGA-3'

Other clones with same target sequence:

(none)