Construct: shRNA TRCN0000417793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007139.2-1753s21c1
DNA Barcode:
TACTAAAGAGAAACTACAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF92 (168374)
Gene Description:
zinc finger protein 92
Transcript:
RefSeq NM_007139.2 (NON-CURRENT)
Match location:
Position 1753 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000417793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTAAAGAGAAACTACAAAC
Hairpin Sequence:
5'-CCGG-TACTAAAGAGAAACTACAAAC-CTCGAG-GTTTGTAGTTTCTCTTTAGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTAAAGAGAAACTACAAACCTCGAGGTTTGTAGTTTCTCTTTAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTAAAGAGAAACTACAAACCTCGAGGTTTGTAGTTTCTCTTTAGTA-3'

Other clones with same target sequence:

(none)